Skip to content

Lactobacillus delbrueckii subsp. bulgaricus

Lactobacillus delbrueckii subsp. bulgaricus suppliers and sourcing guide

Lactobacillus delbrueckii subsp. bulgaricus is a thermophilic lactic acid bacterium and, with Streptococcus thermophilus, one of the two characterizing cultures of yogurt. It is grown by fermentation and sold mainly as frozen or freeze-dried starter culture, usually pre-blended with S. thermophilus, dosed in units per volume of milk. Supplement brands also buy single-strain freeze-dried powder at a stated CFU per gram.

Also known as
Lactobacillus bulgaricus · L. bulgaricus · yogurt culture
Facts checked
November 2026

Forms and grades of lactobacillus delbrueckii subsp. bulgaricus

Suppliers sell different products under the same name. Specify the one you need.

  • Yogurt starter blend

    Frozen pellets or freeze-dried direct-to-vat cultures combining L. bulgaricus and S. thermophilus, sold in units per volume of milk and chosen for acidification speed, texture and post-acidification.

  • Single-strain freeze-dried powder

    L. bulgaricus alone at a stated CFU per gram for supplements or for building custom culture blends.

  • Bulk starter

    Mother cultures for plants that propagate their own bulk starter in milk before inoculating the main vat.

  • Dairy-free grades

    Cultures grown and dried without milk-derived media for plant-based yogurt alternatives, with the allergen status stated on the specification.

What to check on the COA

Compare each result with your own specification when the lot arrives.

Viable count or activity
CFU per gram by plate count (for example on MRS agar at pH 5.4 under anaerobic incubation), or for starter cultures an activity test that measures acidification of milk over a set time and temperature.
Identity
Species and subspecies confirmed by 16S rRNA sequencing or species-specific PCR, with the strain confirmed by a strain-level method against the supplier's deposit.
Phage resistance
For starter cultures, the supplier's phage-resistance or rotation information, since bacteriophage attacks are a common cause of slow or failed dairy fermentations.
Contaminant microbiology
Enterobacteriaceae or coliforms, yeast and mold, Staphylococcus aureus, and absence of Salmonella and Listeria monocytogenes.
Water activity or moisture
Water activity or loss on drying for freeze-dried grades, held to a low maximum because viability drops faster in moister powder.

Storage, lots and expiry

Storage and shelf life

Frozen pellet cultures are held at about -45 °C or colder and freeze-dried cultures frozen or refrigerated, per the supplier's specification, in sealed moisture- and oxygen-barrier packs. Keep cultures in the cold chain until use, let freeze-dried packs warm unopened to avoid condensation, and use opened packs at once. Shelf life is set by the supplier from activity or count stability data at a stated temperature.

Lots and expiry dates

Record the activity or CFU result, strain or blend code and expiry for each lot, and log fermentation times so slow lots can be traced. Rotate blends and stock FEFO, and keep phage-rotation schedules from the supplier with the lot records.

Regulatory status

Facts from regulators and standards bodies, each linked to its source.

  • 21 CFR 131.200 defines yogurt as made with a characterizing bacterial culture containing Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus.

    Source: ecfr.gov (opens in a new tab)
  • Health Canada's Probiotics monograph for natural health products lists Lactobacillus delbrueckii subsp. bulgaricus as a medicinal ingredient, and the licence application and label must give the strain designation.

    Source: webprod.hc-sc.gc.ca (opens in a new tab)
  • 7 CFR 205.605(a) lists microorganisms (any food grade bacteria, fungi and other microorganism) as nonsynthetic substances allowed in processed products labeled organic.

    Source: ecfr.gov (opens in a new tab)

This is general information, not regulatory advice. Status differs by country and by how the ingredient is used, so check the rules for your product and market.

Lactobacillus delbrueckii subsp. bulgaricus suppliers

Manufacturers and distributors whose own websites say they sell it.

  • Sacco System

    Manufacturer · Cadorago, Italy

    Italian group producing lactic and probiotic cultures, starter cultures and rennet, supplying probiotic strains as single ingredients or blends to supplement and food makers.

    • ISO 9001
    • ISO 22000
    • FSSC 22000
    • Kosher
    • Halal

Suppliers are in alphabetical order and none pays to appear. Each claim is checked against the supplier’s own website on the date shown. Ask for the specification sheet and a recent COA before you order, and confirm details with the supplier.

Frequently asked questions

What forms of lactobacillus delbrueckii subsp. bulgaricus can brands buy?

Common forms include yogurt starter blend, single-strain freeze-dried powder, bulk starter and dairy-free grades. Ask each supplier for the specification sheet of the exact grade they quote.

What should the COA for lactobacillus delbrueckii subsp. bulgaricus include?

Expect viable count or activity, identity, phage resistance, contaminant microbiology and water activity or moisture, each with its method, specification and result for that lot.

How should lactobacillus delbrueckii subsp. bulgaricus be stored?

Frozen pellet cultures are held at about -45 °C or colder and freeze-dried cultures frozen or refrigerated, per the supplier's specification, in sealed moisture- and oxygen-barrier packs. Keep cultures in the cold chain until use, let freeze-dried packs warm unopened to avoid condensation, and use opened packs at once. Shelf life is set by the supplier from activity or count stability data at a stated temperature.

What is the regulatory status of lactobacillus delbrueckii subsp. bulgaricus?

21 CFR 131.200 defines yogurt as made with a characterizing bacterial culture containing Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Health Canada's Probiotics monograph for natural health products lists Lactobacillus delbrueckii subsp. bulgaricus as a medicinal ingredient, and the licence application and label must give the strain designation. 7 CFR 205.605(a) lists microorganisms (any food grade bacteria, fungi and other microorganism) as nonsynthetic substances allowed in processed products labeled organic.

Sell ingredients to brands?

Request a free listing for the ingredients you make or distribute. No paid placement.

Request a listing