- Viable count or activity
- CFU per gram by plate count (for example on MRS agar at pH 5.4 under anaerobic incubation), or for starter cultures an activity test that measures acidification of milk over a set time and temperature.
- Identity
- Species and subspecies confirmed by 16S rRNA sequencing or species-specific PCR, with the strain confirmed by a strain-level method against the supplier's deposit.
- Phage resistance
- For starter cultures, the supplier's phage-resistance or rotation information, since bacteriophage attacks are a common cause of slow or failed dairy fermentations.
- Contaminant microbiology
- Enterobacteriaceae or coliforms, yeast and mold, Staphylococcus aureus, and absence of Salmonella and Listeria monocytogenes.
- Water activity or moisture
- Water activity or loss on drying for freeze-dried grades, held to a low maximum because viability drops faster in moister powder.