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Tannase

Tannase suppliers and sourcing guide

Tannase is an enzyme that splits the ester bonds in hydrolyzable tannins and catechin gallates, releasing gallic acid. It is made by fermenting molds such as Aspergillus oryzae or Aspergillus niger, then filtering, concentrating and drying or formulating the enzyme. Beverage and extract makers use it mainly in tea processing to limit cloudiness (cream-down) in ready-to-drink and instant tea, and in juice and botanical extracts.

Category
Enzymes
Also known as
Tannin acylhydrolase · Tannin acyl hydrolase · EC 3.1.1.20
Facts checked
November 2026

Forms and grades of tannase

Suppliers sell different products under the same name. Specify the one you need.

  • Powder

    Dried tannase on a carrier such as dextrin, used in tea leaf and tea extract processing.

  • Liquid concentrate

    Stabilized liquid tannase for dosing into tea or juice extracts.

  • Immobilized tannase

    Enzyme bound to a solid carrier for continuous processing, used mainly by large extract makers.

What to check on the COA

Compare each result with your own specification when the lot arrives.

Tannase activity
Activity by hydrolysis of a gallate ester such as methyl gallate or tannic acid, in the supplier's units per gram.
Production strain and antibiotic activity
Absence of the production organism and of antibiotic activity in the finished preparation, as FCC asks for microbial enzymes; ask for a statement of the strain and whether it is genetically modified.
Loss on drying
Moisture for powders by loss on drying; enzyme activity holds up better in dry product, so moisture above spec is a warning sign for activity loss in storage.
Heavy metals
Lead (and often arsenic, cadmium and mercury) by ICP-MS, reported against FCC enzyme-preparation limits or the supplier's specification.
Microbiology
Total plate count, coliforms, E. coli and Salmonella, reported against the Food Chemicals Codex (FCC) general limits for enzyme preparations or the supplier's tighter specification.
Mycotoxins
Aflatoxin testing where the production organism is an Aspergillus species, if the supplier's specification includes it.

Storage, lots and expiry

Storage and shelf life

Enzyme powders lose activity with heat and moisture, so keep them sealed in the original lined container in a cool, dry place, and refrigerate liquid preparations if the supplier says to. Shelf life is set by each supplier's specification, usually as a guaranteed minimum activity to a stated date, and older stock is retested for activity before use. Supplier safety data sheets for enzyme powders call for dust control and respiratory protection when weighing and blending.

Lots and expiry dates

Activity varies from lot to lot, so record the measured activity and its unit for each lot and set the dose from it rather than from the nominal value. Record the production organism, carrier and any stabilizer or preservative, and rotate stock first-expired, first-out.

Regulatory status

Facts from regulators and standards bodies, each linked to its source.

  • GRN 1192, for tannase produced by Aspergillus oryzae (Mitsubishi Chemical), for use in tea-based beverages, juices, soft drinks, flavored water, jelly and botanical extracts, closed with FDA having no questions.

    Source: hfpappexternal.fda.gov (opens in a new tab)

This is general information, not regulatory advice. Status differs by country and by how the ingredient is used, so check the rules for your product and market.

Tannase suppliers

Manufacturers and distributors whose own websites say they sell it.

  • Amano Enzyme

    Manufacturer · Nagoya, Japan

    Japanese enzyme maker founded in 1899 that supplies digestive enzymes for dietary supplements and processing enzymes for food and beverage production.

    • SQF
    • Kosher
    • Halal

Suppliers are in alphabetical order and none pays to appear. Each claim is checked against the supplier’s own website on the date shown. Ask for the specification sheet and a recent COA before you order, and confirm details with the supplier.

Frequently asked questions

What forms of tannase can brands buy?

Common forms include powder, liquid concentrate and immobilized tannase. Ask each supplier for the specification sheet of the exact grade they quote.

What should the COA for tannase include?

Expect tannase activity, production strain and antibiotic activity, loss on drying, heavy metals, microbiology and mycotoxins, each with its method, specification and result for that lot.

How should tannase be stored?

Enzyme powders lose activity with heat and moisture, so keep them sealed in the original lined container in a cool, dry place, and refrigerate liquid preparations if the supplier says to. Shelf life is set by each supplier's specification, usually as a guaranteed minimum activity to a stated date, and older stock is retested for activity before use. Supplier safety data sheets for enzyme powders call for dust control and respiratory protection when weighing and blending.

What is the regulatory status of tannase?

GRN 1192, for tannase produced by Aspergillus oryzae (Mitsubishi Chemical), for use in tea-based beverages, juices, soft drinks, flavored water, jelly and botanical extracts, closed with FDA having no questions.

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