- Steviol glycoside assay
- Individual glycosides (Reb A, Reb M, Reb D, stevioside and others) and total steviol glycosides by HPLC, confirming the purity grade.
- Loss on drying and ash
- Moisture and mineral residue, which affect purity calculations and handling.
- Residual solvents
- Methanol and ethanol from purification, tested by GC and checked against limits in food additive specifications.
- Heavy metals
- Lead and arsenic at minimum, often cadmium and mercury.
- Microbiology
- Total plate count, yeast and mold, coliforms, E. coli and Salmonella.
- Physical properties
- Appearance, solubility, pH of a solution and particle size, which affect blending in powders and beverages.
- Production route
- Statement of leaf extraction, bioconversion or fermentation origin, which matters for labeling and some regulatory approvals.