- Identity and species (HPTLC)
- Fingerprint to confirm the Echinacea species and plant part; also catches substitution with Parthenium integrifolium root, a known adulterant.
- Marker assay (HPLC)
- Cichoric acid, caftaric acid, echinacoside or alkamides, depending on species and spec, by a named HPLC method.
- Heavy metals
- Lead, arsenic, cadmium and mercury by ICP-MS; root material carries more soil contact.
- Microbiology
- Total plate count, yeast and mold, E. coli and Salmonella.
- Pesticide residues
- Multi-residue screen, required for organic claims and common for cultivated material.
- Residual solvents and moisture
- Ethanol or other solvents for extracts against USP <467>; loss on drying for herb and root.