- Beta-glucans (enzymatic)
- Beta-glucans by an enzymatic method, with alpha-glucan reported to flag grain-grown material.
- Identity
- Microscopy, HPTLC or DNA barcoding to confirm Inonotus obliquus and sclerotium rather than mycelium on grain.
- Heavy metals
- Lead, arsenic, cadmium and mercury by ICP-MS; wild material reflects the harvest area.
- Microbiology
- Total plate count, yeast and mold, E. coli and Salmonella.
- Oxalate content
- Some buyers test oxalate in powders and extracts, since chaga is known to carry high levels.
- Moisture and foreign matter
- Loss on drying, plus wood and bark fragments in chunks and powder.