- Identity
- Macroscopic and microscopic examination or HPTLC fingerprint to confirm Hibiscus sabdariffa calyces.
- Anthocyanins and acidity
- Total anthocyanins by pH-differential UV-Vis, and acidity by titration; these set color and tartness in the finished product.
- Heavy metals
- Lead, arsenic, cadmium and mercury by ICP-MS against the buyer's limits and any Prop 65 exposure targets.
- Microbiology
- Total plate count, yeast and mold, E. coli and Salmonella. Raw cut herb and powder carry more microbial load than extracts and may be steam treated.
- Pesticide residues
- Multi-residue screen by GC-MS/MS and LC-MS/MS, required for organic claims and commonly asked for on conventional lots.
- Physical tests
- Loss on drying, foreign matter (seeds, stems, stones), particle size for cut and powder, and color strength for extracts.