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Whey protein hydrolysate

Whey protein hydrolysate suppliers and sourcing guide

Whey protein hydrolysate is whey protein concentrate or isolate that has been partly broken down into peptides with food-grade protease enzymes, then heat treated to stop the enzymes, filtered and spray dried. Suppliers describe it by degree of hydrolysis (DH), the share of peptide bonds cut, and by its peptide size profile. Higher DH gives a thinner, more soluble powder with a more bitter taste, so brands usually buy a specific DH for their flavor system.

Also known as
WPH · hydrolyzed whey protein · hydrolyzed whey · whey peptides
Facts checked
November 2026

Forms and grades of whey protein hydrolysate

Suppliers sell different products under the same name. Specify the one you need.

  • Partially hydrolyzed

    Low degree of hydrolysis (often under 10%), close to intact whey in taste and used in ready-to-mix powders and drinks.

  • Extensively hydrolyzed

    High degree of hydrolysis with mostly small peptides; more soluble and more bitter, used in specialty nutrition products.

  • Isolate-based

    Hydrolysate made from whey protein isolate, with higher protein and lower lactose and fat than concentrate-based grades.

  • Debittered or flavor-masked

    Grades treated or blended to reduce bitterness, sometimes with added flavor or sweetener carriers that belong on the spec.

What to check on the COA

Compare each result with your own specification when the lot arrives.

Protein (Kjeldahl or Dumas)
Total nitrogen times 6.38, reported as-is and on a dry basis.
Degree of hydrolysis
Percent DH by OPA or TNBS method, or a molecular weight distribution by size-exclusion HPLC; this is the main spec that separates one hydrolysate from another.
Moisture, fat, ash and lactose
Loss on drying, fat, ash and lactose as for whey concentrate or isolate, depending on the starting material.
Microbiology
Total plate count, coliforms, yeast and mold, Salmonella, Listeria and Staphylococcus, plus Cronobacter for infant or medical uses.
Heavy metals
Lead, arsenic, cadmium and mercury by ICP-MS against the buyer's supplement or food limits.

Storage, lots and expiry

Storage and shelf life

Hydrolysates are more hygroscopic than intact whey and can cake or turn sticky in humid conditions, so keep bags sealed and stored cool and dry. Warm storage speeds browning and flavor change. Shelf life follows the supplier's specification, with a retest of moisture, micro and taste for aged stock.

Lots and expiry dates

Degree of hydrolysis and bitterness can shift between lots, so record DH, protein and a taste check for each lot before it goes into a flavored product. Keep a retained sample from each lot for sensory comparison.

Regulatory status

Facts from regulators and standards bodies, each linked to its source.

This is general information, not regulatory advice. Status differs by country and by how the ingredient is used, so check the rules for your product and market.

Whey protein hydrolysate suppliers

Manufacturers and distributors whose own websites say they sell it.

  • Actus Nutrition

    Manufacturer · Eden Prairie, MN

    US dairy ingredient maker, formerly Milk Specialties Global, producing whey proteins, milk protein concentrates and isolates, and caseins and caseinates for food and nutrition brands.

Suppliers are in alphabetical order and none pays to appear. Each claim is checked against the supplier’s own website on the date shown. Ask for the specification sheet and a recent COA before you order, and confirm details with the supplier.

Frequently asked questions

What forms of whey protein hydrolysate can brands buy?

Common forms include partially hydrolyzed, extensively hydrolyzed, isolate-based and debittered or flavor-masked. Ask each supplier for the specification sheet of the exact grade they quote.

What should the COA for whey protein hydrolysate include?

Expect protein (Kjeldahl or Dumas), degree of hydrolysis, moisture, fat, ash and lactose, microbiology and heavy metals, each with its method, specification and result for that lot.

How should whey protein hydrolysate be stored?

Hydrolysates are more hygroscopic than intact whey and can cake or turn sticky in humid conditions, so keep bags sealed and stored cool and dry. Warm storage speeds browning and flavor change. Shelf life follows the supplier's specification, with a retest of moisture, micro and taste for aged stock.

What is the regulatory status of whey protein hydrolysate?

Health Canada's Workout Supplements monograph lists whey protein hydrolysate with cow and goat milk (Bos taurus, Capra hircus) as the source material. 21 CFR 102.22 requires the common or usual name of a protein hydrolysate to include the identity of the food source the protein came from.

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